Journal: Journal of Neuroinflammation

Article Title: Sex specific effects of a high fat diet on metabolism, cognition, and pathology in the Tg-SwDI mouse model of Alzheimer’s disease

doi: 10.1186/s12974-026-03719-0

Figure Lengend Snippet: Diet affects weight, visceral adiposity, and glucose tolerance in WT and Tg-SwDI males and females. A The experimental timeline is shown. B , C Body weight prior to the GTT and following 6 months of dietary intervention was used to assess weight gain. D , E Wet weight (g) of visceral fat was assessed at the end of the experiment and analyzed relative to percent of total body weight at that time. F - I GTT was performed to assess metabolism and diabetic state. F , G After overnight fasting (t = 0), blood glucose levels were measured, and mice were injected with a glucose challenge during which blood glucose was measured at 15, 30, 60, 90, and 120 min. Each group was compared to the control WT LFD group at each time point, such that significantly different blood glucose compared to control is denoted as * p < 0.05 WT LFD vs. WT HFD, # p < 0.05 WT LFD vs. TgSwDI LFD, and ^ p < 0.05 WT LFD vs. TgSwDI HFD. H , I Area under the curve (AUC) from GTT was assessed as a measure of responsiveness to the glucose challenge. Higher AUC is indicative of worse metabolic disease. Error bars represent SEM. * p < 0.05; ** p < 0.01; **** p < 0.0001. Two-way ANOVA ( n = 11–20/group). Main effects are demonstrated above the graph when significant, and post-hoc comparisons are demonstrated as significance bars where appropriate

Article Snippet: Mice from the Tg-SwDI strain were obtained from the Mutant Mouse Resource and Research Center at the Jackson Laboratory, an NIH-funded strain repository, and were donated to the center by William Van Nostrand, Ph.D., Stony Brook University.

Techniques: Injection, Control

Journal: Journal of Neuroinflammation

Article Title: Sex specific effects of a high fat diet on metabolism, cognition, and pathology in the Tg-SwDI mouse model of Alzheimer’s disease

doi: 10.1186/s12974-026-03719-0

Figure Lengend Snippet: Diet and sex interact to affect anxiety-like behavior, general activity, and recognition memory. A - D Anxiety-like behavior was assessed through percent of time spent in the corners ( A , B ) and in the center ( C , D ) during the open field test. Percent of time spent in the corners was increased in WT and Tg-SwDI animals receiving HFD across sexes, and similarly percent of time in the center was decreased in WT and TgSwDI animals receiving HFD. E , F Distance traveled during the open field test was used to assess general locomotor activity. HFD and AD independently decreased the total distance traveled in both males ( E ) and females ( F ). G , H Recognition memory was assessed using the novel object recognition test (NORT). Percent of time spent with the novel object relative to total time spent with objects was calculated, such that performance significantly greater than 50% chance is indicative of intact memory. All AD males and HFD AD females did not perform significantly greater than chance. Pink and blue line = chance. Error bars represent SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05 vs. chance, ## p < 0.01 vs. chance, ### p < 0.001 vs. chance. Two-way ANOVA and one sample t-test ( n = 13–20/group). Main effects are demonstrated above the graph when significant, and post-hoc comparisons are demonstrated as significance bars where appropriate

Article Snippet: Mice from the Tg-SwDI strain were obtained from the Mutant Mouse Resource and Research Center at the Jackson Laboratory, an NIH-funded strain repository, and were donated to the center by William Van Nostrand, Ph.D., Stony Brook University.

Techniques: Activity Assay

Journal: Journal of Neuroinflammation

Article Title: Sex specific effects of a high fat diet on metabolism, cognition, and pathology in the Tg-SwDI mouse model of Alzheimer’s disease

doi: 10.1186/s12974-026-03719-0

Figure Lengend Snippet: HFD impairs spatial memory in female Tg-SwDI animals. Spatial learning and memory were assessed using the Barnes maze. Spatial memory was assessed through the probe trial ( A - D ) through percent of time in the cone adjacent to the target, such that performance above 15% chance was indicative of greater learning,, B ) and percent of entries in the incorrect holes relative to the correct hole ( C , D ). TgSwDI females spent less time in the target cone ( F ) and had greater percentage of erroneous entries ( H ) during the probe trial compared to WT females. Red line = chance. Error bars represent SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05 vs. chance, ## p < 0.01 vs. chance, ### p < 0.001 vs. chance. Two-way ANOVA and one sample t-test or Wilcoxon test if not normally distributed ( n = 14–20/group). Main effects are demonstrated above the graph when significant, and post-hoc comparisons are demonstrated as significance bars where appropriate

Article Snippet: Mice from the Tg-SwDI strain were obtained from the Mutant Mouse Resource and Research Center at the Jackson Laboratory, an NIH-funded strain repository, and were donated to the center by William Van Nostrand, Ph.D., Stony Brook University.

Techniques: